Inhibition of lymphocyte proliferation by amantadine and its isomer, 2-aminoadamantane; impact on Lyt-2+ T cells while sparing L3T4+ cells
Identifieur interne : 002021 ( Main/Exploration ); précédent : 002020; suivant : 002022Inhibition of lymphocyte proliferation by amantadine and its isomer, 2-aminoadamantane; impact on Lyt-2+ T cells while sparing L3T4+ cells
Auteurs : Connie Clark [États-Unis] ; Mildred M. Woodson [États-Unis] ; Herbert T. Nagasawa [États-Unis]Source :
- Immunopharmacology [ 0162-3109 ] ; 1991.
English descriptors
- Teeft :
- Ablate inhibition, Adamantane, Adamantane derivatives, Adamantane ring, Amantadine, Amantadine hydrochloride, American type culture collection, Amine moiety, Amino, Amino group, Aminoadamantanes, Amtd, Analog, Antibody reagents, Becton dickinson, Biochem pharmacol, Biochim biophys acta, Cell biol, Cell subpopulation, Cell subpopulations, Cell subsets, Cellular uptake, Control response, Corresponding analogs, Ctll, Ctll cells, Culture medium, Cytotoxic, Data show, Dominant role, Drug sensitivity, Gain information, Grand island, Human lymphocytes, Immunol, Immunol today, Independent experiments, Influenza, Influenza illness, Inhibitory, Inhibitory activity, Inhibitory concentration, Inhibitory effect, Isomeric aminoadamantanes, Lymphocyte, Lymphocyte activity, Lymphocyte line, Lymphocyte proliferation, Membrane filtration, Mitogen, Mitogen responses, Mitogeninduced proliferation, Molecular features, Mountain view, Murine cytotoxic, Nitro, Nitro adamantanes, Nitro group, Proliferation, Proliferative, Proliferative response, Proliferative responses, Ratcsf, Receptor, Receptor expression, Recombinant interleukin proliferation, Rimantadine, Sigma chemical, Spleen, Spleen cells, Standard error, Subpopulation, Substituent group, Successful treatment, Tritiated thymidine, Various concentrations, Veterans affairs.
Abstract
Abstract: The present in vitro investigations on amantadine (AmTd) and its isomer 2-aminoadamantane (2-NH2-Adam), and the corresponding analogs, 1-nitroadamantane (1-NO2-Adam) and 2-nitroadamantane (2-NO2-Adam), were undertaken to gain information about molecular features that might have a dominant role in inhibiting T lymphocyte proliferation and to determine whether all, or a subpopulation of thymic-dependent (T) lymphocytes might be impacted by these drugs. Studies were done using lymphocytes from untreated normal mice as well as cloned murine cytotoxic T lymphocytes, CTLL cells. T lymphocytes were defined by their proliferative response to concanavalin A (Con A), and thymic-independent (B) lymphocytes by their proliferative response lipopolysaccharide (LPS). Proliferation of CTLL cells was induced by supplementing the culture medium with lymphokine-containing medium or by adding recombinant interleukin (IL)-2. Proliferation was assessed by quantifying cellular incorporation of tritiated thymidine. The data show that the aminoadamantanes, AmTd and 2-NH2-Adam, impacted on Lyt-2+ T lymphocytes while sparing L3T4+ T lymphocytes. In addition, the data show that the location of the substituent group on the adamantane ring altered the molecule's capacity to modulate lymphocyte activity. And finally, results of studies comparing the inhibitory activity of AmTd and 1-NO2-Adam suggest a non-lysosomal mechanism of action. Possible implications of these findings are discussed.
Url:
DOI: 10.1016/0162-3109(91)90006-K
Affiliations:
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Nagasawa</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Medical Research Division, Department of Veterans Affairs Medical Center, Minneapolis, Minnesota</wicri:regionArea><placeName><region type="state">Minnesota</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Immunopharmacology</title><title level="j" type="abbrev">IMPHAR</title><idno type="ISSN">0162-3109</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1991">1991</date><biblScope unit="volume">21</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="41">41</biblScope><biblScope unit="page" to="50">50</biblScope></imprint><idno type="ISSN">0162-3109</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0162-3109</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Ablate inhibition</term><term>Adamantane</term><term>Adamantane derivatives</term><term>Adamantane ring</term><term>Amantadine</term><term>Amantadine hydrochloride</term><term>American type culture collection</term><term>Amine moiety</term><term>Amino</term><term>Amino group</term><term>Aminoadamantanes</term><term>Amtd</term><term>Analog</term><term>Antibody reagents</term><term>Becton dickinson</term><term>Biochem pharmacol</term><term>Biochim biophys acta</term><term>Cell biol</term><term>Cell subpopulation</term><term>Cell subpopulations</term><term>Cell subsets</term><term>Cellular uptake</term><term>Control response</term><term>Corresponding analogs</term><term>Ctll</term><term>Ctll cells</term><term>Culture medium</term><term>Cytotoxic</term><term>Data show</term><term>Dominant role</term><term>Drug sensitivity</term><term>Gain information</term><term>Grand island</term><term>Human lymphocytes</term><term>Immunol</term><term>Immunol today</term><term>Independent experiments</term><term>Influenza</term><term>Influenza illness</term><term>Inhibitory</term><term>Inhibitory activity</term><term>Inhibitory concentration</term><term>Inhibitory effect</term><term>Isomeric aminoadamantanes</term><term>Lymphocyte</term><term>Lymphocyte activity</term><term>Lymphocyte line</term><term>Lymphocyte proliferation</term><term>Membrane filtration</term><term>Mitogen</term><term>Mitogen responses</term><term>Mitogeninduced proliferation</term><term>Molecular features</term><term>Mountain view</term><term>Murine cytotoxic</term><term>Nitro</term><term>Nitro adamantanes</term><term>Nitro group</term><term>Proliferation</term><term>Proliferative</term><term>Proliferative response</term><term>Proliferative responses</term><term>Ratcsf</term><term>Receptor</term><term>Receptor expression</term><term>Recombinant interleukin proliferation</term><term>Rimantadine</term><term>Sigma chemical</term><term>Spleen</term><term>Spleen cells</term><term>Standard error</term><term>Subpopulation</term><term>Substituent group</term><term>Successful treatment</term><term>Tritiated thymidine</term><term>Various concentrations</term><term>Veterans affairs</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The present in vitro investigations on amantadine (AmTd) and its isomer 2-aminoadamantane (2-NH2-Adam), and the corresponding analogs, 1-nitroadamantane (1-NO2-Adam) and 2-nitroadamantane (2-NO2-Adam), were undertaken to gain information about molecular features that might have a dominant role in inhibiting T lymphocyte proliferation and to determine whether all, or a subpopulation of thymic-dependent (T) lymphocytes might be impacted by these drugs. Studies were done using lymphocytes from untreated normal mice as well as cloned murine cytotoxic T lymphocytes, CTLL cells. T lymphocytes were defined by their proliferative response to concanavalin A (Con A), and thymic-independent (B) lymphocytes by their proliferative response lipopolysaccharide (LPS). Proliferation of CTLL cells was induced by supplementing the culture medium with lymphokine-containing medium or by adding recombinant interleukin (IL)-2. Proliferation was assessed by quantifying cellular incorporation of tritiated thymidine. The data show that the aminoadamantanes, AmTd and 2-NH2-Adam, impacted on Lyt-2+ T lymphocytes while sparing L3T4+ T lymphocytes. In addition, the data show that the location of the substituent group on the adamantane ring altered the molecule's capacity to modulate lymphocyte activity. And finally, results of studies comparing the inhibitory activity of AmTd and 1-NO2-Adam suggest a non-lysosomal mechanism of action. Possible implications of these findings are discussed.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Minnesota</li></region></list><tree><country name="États-Unis"><region name="Minnesota"><name sortKey="Clark, Connie" sort="Clark, Connie" uniqKey="Clark C" first="Connie" last="Clark">Connie Clark</name></region><name sortKey="Nagasawa, Herbert T" sort="Nagasawa, Herbert T" uniqKey="Nagasawa H" first="Herbert T." last="Nagasawa">Herbert T. Nagasawa</name><name sortKey="Woodson, Mildred M" sort="Woodson, Mildred M" uniqKey="Woodson M" first="Mildred M." last="Woodson">Mildred M. Woodson</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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